Verified email at kmu.ac.ir
Kerman University of Medical Sciences, Kerman,Iran
Leishmaniasis, toxoplasmosis, Bioteqnology ,Giardiasis
Razieh Tavakoli Oliaee, Iraj Sharifi, Mehdi Bamorovat, Alireza Keyhani, Zahra Babaei, Ehsan Salarkia, Rahele Tavakoly, Ahmad Khosravi, Mahshid Mostafavi, Fatemeh Sharifi, and Seyed Mohammad Mousavi
International Immunopharmacology, ISSN: 15675769, eISSN: 18781705, Published: September 2020 Elsevier BV
Hajar Shabandoust, Iraj Sharifi, Omid Raiesi, Muhammad Ibrahim Getso, Ebrahim Dezaki Saedi, Ali Afgar, Giti Shirvani, Ehsan Salarkia, and Zahra Babaei
Journal of Parasitic Diseases, ISSN: 09717196, eISSN: 09750703, Pages: 40-48, Published: 1 March 2020 Springer Science and Business Media LLC
Leishmania, an obligate intracellular parasite is eliminated by a strong Th-1 host response. As Vitamin D metabolism and its receptor activity are important factors in human native immune system against some microorganisms, we hypothesized that VDR gene polymorphisms and concentration of Vitamin D might have effect on incidence of cutaneous leishmaniasis. The aim of this study was to investigate the association between VDR gene polymorphism and/or the serum vitamin D level and leishmaniasis in the infected patients in comparison to the healthy individuals. In this case–control study, the BsmI, FokI and Taq1 polymorphisms in the VDR gene and serum levels of vitamin D were studied in Iranian infected with Leishmania tropica (n = 50) and healthy controls using polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) and Electrochemiluminescence methods respectively. Data were statistically analyzed using SPSS software, Chi square and ANOVA tests. The results of this study showed that despite the relatively higher frequency of BsmI-BB, FokI-FF and TaqI-Tt than Non BsmI-BB, Non FokI-FF and Non TaqI-Tt in the patients compared with the healthy individuals, the differences were not statistically significant (P > 0.05). Based on our findings, the relationship between the VDR polymorphism, the serum concentration of 25-hydroxyvitamin D and the susceptibility to Leishmania tropica infection, remains unclear requiring further in-depth studies. However, for better interpretation, it is necessary to consider factors such as the size of the sample examined and the other alleles of VDR, including ApaI.
Giti Shirvani, Majid Fasihi-Harandi, Omid Raiesi, Nasrin Bazargan, Mohammad Javad Zahedi, Iraj Sharifi, Behjat Kalantari-Khandani, Maryam Nooshadokht, Hajar Shabandoust, Mohammad Ali Mohammadi, Mohammad Ebrahimipour, and Zahra Babaei
Acta Parasitologica, ISSN: 12302821, eISSN: 18961851, Pages: 90-96, Published: 1 March 2020 Walter de Gruyter GmbH
Blastocystis is a parasite that colonizes in the human intestine. Its clinical features include diarrhea, abdominal pain, or urticarial and irritable bowel syndrome (IBS). Spite of being significant genetic diversity and numerous subtypes within the genus there were no associations between its subtypes and symptomatology. Aim of this project was subtyping of the protozoa in 184 Iranian people with history of IBS/IBD (n = 74) or chronic urticaria (n = 59) and individuals referred to general clinic (n = 51). Microscopic and molecular examinations used for identifying and subtyping of Blastocystis. Overall, frequency of the parasite was 24.46% while, 29.41% of people who referred to general clinic, 20.27%, and 25.42% of IBS/IBD and urticarial cases were infected, respectively. Subtyping result showed that 28.89% of all people were infected with Blastocystis sp. while the prevalence of ST3, ST2 and ST1 were 22.22%, 22.22%, and 17.78%, respectively. Blastocystis sp., was identified in most IBS/IBD cases (46.7%) followed with ST2 and ST3 (13.3 and 13.3, respectively). Whereas, in chronic urticaria group ST2(33.3%) was the major subtype and most individuals in control group were infected with ST3 (33.3%). Pearson’s Chi Square test showed no significant differences between the parasite or subtype prevalence and diseases (p > 0.05). Given significant factors have effect on clinical signs including host or parasite genetics, microbiota, as well as environmental factors, it seems that further studies are needed to find out different markers of host susceptibility to diverse parasite genotypes in patients with irritable bowel syndrome or urticaria.
Iranian Journal of Public Health, ISSN: 22516085, eISSN: 22516093, Pages: 351-359, Published: February 2020
Zahra Babaei, Iman Pouladi, Abbass Ashtari, Gasem Azimi, and Mohammad Niakan
Jundishapur Journal of Microbiology, ISSN: 20083645, eISSN: 20084161, Published: 2020 Kowsar Medical Institute
Zahra Babaei, Reza Taherkhani, Afshin Barazesh, Marzieh Taherzadeh, Soleiman Khorami, and Moradali Fouladvand
Journal of Parasitic Diseases, ISSN: 09717196, eISSN: 09750703, Published: 2020 Springer Science and Business Media LLC
Hydatid cyst is one of the parasitic zoonoses caused by infection with the larval stage of Echinococcus granulosus tapeworm. The spread of this parasite is global and is of great importance in terms of public health. To date, ten different species of this parasite have been identified that differ in characteristics such as life cycle, epidemiology and pathogenesis. The purpose of this study was to determine the genotype and phylogenetic relationship of hydatid cysts isolated from livestock of Bushehr province, Iran. About 62 samples of hepatic and pulmonary hydatid cysts were collected from slaughtered animals. DNA extracted by phenol–chloroform method was amplified by PCR using primers specific for the cox1 gene. The PCR products of 50 samples were sequenced and analyzed using BioEdit software and compared with sequences in the GenBank. The phylogenetic tree was drawn using Neighbor Joining tree-NJ method, and its reliability was evaluated. Sequencing results showed that out of 50 sequenced samples, 43 samples had the genotype of Echinococcus granulosus and 7 samples had the genotype of Taenia hydatigena. By drawing a phylogenetic tree, all 43 hydatid cyst samples belonged to G1 strain. The predominance of G1 strain of hydatid cyst in livestock of Bushehr province shows the main role of this genotype in establishing the life cycle of parasite in this region and if the genotype of the parasite in dogs and humans is determined, then these findings can be used to disrupt the life cycle of the parasite and reduce the human infections.
I. Sharifi, F. Tabatabaie, S. Nikpour, M. Mostafavi, Razieh Tavakoli Oliaee, F. Sharifi, Z. Babaei, Elham Jafari, Ehsan Salarkia and D. Shahbazzadeh
Acta Parasitologica, ISSN: 12302821, eISSN: 18961851, Published: 2020
PURPOSE The aim of this study was to explore the activity of Naja naja oxiana venom on Leishmania tropica and its modes of action. METHODS Different fractions of Naja naja oxiana venom (NNOV) were prepared and characterized by high-performance liquid chromatography. The superior component, fraction k (FK) was selected. The activity of the fraction was assessed using advanced assays. RESULTS Interleukin (IL)-12, TNF-α and iNOS gene expression as the indicators of Th1 significantly increased. In contrast, the level of IL-10, as the marker of T helper 2 substantially decreased (p < 0.001). Reactive oxygen species (ROS) detection showed a significant increase (p < 0.001) after treatment with different concentrations of NNOV-FK, unlike arginase (L-ARG) activity which showed a significant reduction (p < 0.001). The NNOV-FK showed significant lethal activity on the L. tropica stages. CONCLUSION The findings demonstrated that NNOV-FK represented a strong leishmanicidal activity on L. tropica stages. The major modes of NNOV-FK action are multidimensional, which perceives the induction of a synergistic response and upregulation of the immune-modulatory role towards Th1 response against L. tropica stages as well as apoptotic and anti-metabolic action as a model drug to generate ROS, block the polyamine synthesis and lead to parasite death.
Morteza SADUQI, Iraj SHARIFI, Zahra BABAEI, Alireza KEYHANI, Mahshid MOSTAFAVI, Maryam HAKIMI PARIZI, Mahdiyeh GHASEMIAN, Mehdi BAMOROVAT, Fatemeh SHARIFI, Mohammad Reza AFLATOONIAN, Fariba SHARIFIFAR, Pooya GHASEMI NEJAD, Ahmad KHOSRAVI, Ehsan SALARKIA, and Rajender S. VARMA
Iranian Journal of Parasitology, ISSN: 17357020, eISSN: 2008238X, Pages: 521-533, Published: October-December 2019 Knowledge E
Background: Pentavalent antimonials such as meglumine antimoniate (MA, Glucantime), are the first-line treatment against leishmaniasis, but at present, they have basically lost their efficacy. This study was aimed to explore epigallocatechin 3-O-gallate (EGCG), alone or in combination with MA against Leishmania tropica stages. Methods: All experiments were carried out in triplicate using colorimetric assay, macrophage model, flow cytometry and quantitative real-time PCR. This experimental study was carried out in 2017 in Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran. Results: Promastigotes and amastigotes were more susceptible to EGCG than MA alone, but the effect was more profound when used in combination. EGCG exhibited high antioxidant level with a remarkable potential to induce apoptosis. Furthermore, the results showed that the level of gene expression pertaining to Th-1 was significantly up-regulated (P<0.001). Conclusion: EGCG demonstrated a potent anti-leishmanial effect alone and more enhanced lethal activity in combination. The principal mode of action entails the stimulation of a synergistic response and up-regulation of the immunomodulatory role towards Th-1 response against L. tropica.
Seyed Zia Razavinasab, Iraj Sharifi, Mohammad Reza Aflatoonian, Zahra Babaei, Mohammad Ali Mohammadi, Ehsan Salarkia, Fatemeh Sharifi, Abbas Aghaei Afshar, and Mehdi Bamorovat
Transboundary and Emerging Diseases, ISSN: 18651674, eISSN: 18651682, Pages: 1602-1610, Published: July 2019 Wiley
Cutaneous leishmaniasis (CL) has increased remarkably in Iran and has expanded into new areas. The present study aimed to assess the emerging CL outbreak in southeastern Iran using high resolution melting-polymerase chain reaction (HRM-PCR) and phylogenetic analysis using the 7SL RNA gene marker. A cross-sectional and analytical survey was conducted during a house-to-house census of 11,021 inhabitants in Narmashir County in southeastern Iran in 2016. The cases were detected by direct smear microscopic examination and sequencing and were characterized using the 7SL RNA gene. All age groups and sexes were equally affected. Most were single lesions (70.7%). The hands (55.2%) and face (37.9%) were the main sites of involvement. The disease was more common among illiterate persons. Sequencing and HRM-PCR revealed that Leishmania tropica (accession no. MH632168 Qale-Shahid) was the principal causative agent of anthroponotic CL (ACL) in new areas of expansion. This is the first emergence of ACL in rural areas of Narmashir County. Based on the molecular data, the causative parasite species confirmed to be L. tropica. Sequencing and phylogenetic analysis indicated that a single clone of the organism derived from a single source has spread into the affected villages. Construction of a main road, population movement and recent urbanization in the area are likely the major factors associated with the establishment of this new outbreak. This study was essential to enable the planning of effective therapeutic and prophylactic measures to control the disease.
Zahra Babaei, Farideh Tohidi, Bahram Kazemi, Mojgan Bandehpour, Iraj Sharifi, MohammadReza Rabiei, and EbrahimSaedi Dezaki
Journal of Vector Borne Diseases, ISSN: 09729062, Pages: 98-104, Published: June 2019 Medknow
Background & objectives: Leishmania parasites cause various clinical symptoms in humans such as cutaneous ulcers and fatal visceral diseases. These parasites cannot synthesize purine rings de novo and must uptake purines from their hosts via salvage. Salvage is regulated by permeases in the cell membrane. There are hundreds of membrane transporter proteins to receive nutrients in Leishmania. Nucleoside transporter 4 (NT4) is one of the purine transporters that is involved in enhancing the uptake of adenine in Leishmania major. They are important new drug targets for the treatment of leishmaniasis because they can be used to transport toxic purine analogs to kill parasitic cells, thus preventing the progression of the infection. The present study was conducted to silence the NT4 nucleobase involved in the salvage pathway to interrupt purine nucleotide membrane transport in the cells of L. major. Methods: In this study, a 502 bp segment of NT4 gene sequence was selected and designed as antisense transcripts after insertion in the parasite. The NT4 construct was transfected into L. major promastigotes for in vitro study of gene expression. Then, BALB/c mice infected with transgenic Leishmania and wild-type strain along with the number and size of lesions were studied in vivo. Results: The study showed that relative expression of NT4 gene in mutant Leishmania was lower than in the control on Day 3 to 20. The percentages and the number of amastigotes in infected macrophages with wild-type strain L. major were more than infected macrophages with mutant parasites. Infected BALB/c mice with transgenic Leish- mania showed a lower number and size of lesions than the BALB/c mice infected with wild-type strain. Interpretation & conclusion: The results of the study indicated that the use of antisense RNA reduced NT4 gene expression in L. major. Further, studies are needed to ascertain that the use of antisense can be considered as a new treatment for leishmaniasis.
Mohammad Reza Aflatoonian, Iraj Sharifi, Behnaz Aflatoonian, Mehdi Bamorovat, Amireh Heshmatkhah, Zahra Babaei, Pooya Ghasemi Nejad Almani, Mohammad Ali Mohammadi, Ehsan Salarkia, Abbas Aghaei Afshar, Hamid Sharifi, Fatemeh Sharifi, Ahmad Khosravi, Mehrdad Khatami, Nasir Arefinia, Alireza Fekri, Saeideh Farajzadeh, Ali Khamesipour, Mehdi Mohebali, Mohammad Mehdi Gouya, Mohammad Reza Shirzadi, and Rajender S. Varma
PLoS Neglected Tropical Diseases, ISSN: 19352727, eISSN: 19352735, Published: June 2019 Public Library of Science (PLoS)
BACKGROUND The control of cutaneous leishmaniasis (CL) is facilitated by knowledge of factors associated with the treatment failures in endemic countries. The aim of this evaluation was to identify the potential risk determinants which might affect the significance of demographic and clinical characteristics for the patients with anthroponotic CL (ACL) and the outcome of meglumine antimoniate (MA) (Glucantime) treatment. METHODOLOGY/PRINCIPAL FINDINGS This current was executed as a cohort spanning over a period of 5 years which centered in southeastern part of Iran. Altogether, 2,422 participants were evaluated and 1,391 eligible volunteer patients with ACL caused by Leishmania tropica were included. Overall, 1,116 (80.2%) patients received MA intraleisionally (IL), once a week for 12 weeks along with biweekly cryotherapy, while 275 (19.8%) patients received MA alone (20 mg/kg/day for 3 weeks) (intramuscular, IM). The treatment failure rate in ACL patients was 11% using IL combined with cryotherapy plus IM alone, whilst 9% and 18.5% by IL along with cryotherapy or IM alone, respectively. Multivariate logistic regression model predicted 5 major associated-risk determinants including male (odds ratio (OR) = 1.54, confidence interval (CI) = 1.079-2.22, p = 0.018), lesion on face (OR = 1.574, CI = 1.075-2.303, p = 0.02), multiple lesions (OR = 1.446, CI = 1.008-2.075, p = 0.045), poor treatment adherence (OR = 2.041, CI = 1.204-3.46, p = 0.008) and disease duration > 4 months (OR = 2.739, CI = 1.906-3.936, p≤0.001). CONCLUSIONS/SIGNIFICANCE The present study is the original and largest cohort of ACL patients who treated with MA. A comprehensive intervention and coordinated action by the health authorities and policy-makers are crucial to make sure that patients strictly follow medical instructions. Early detection and effective therapy < 4 months following the onset of the lesion is critical for successful treatment of the patients. Since a significant number of patients are still refractory to MA, reducing man-vector exposure and development of new effective alternative drugs are essential measures against ACL due to L. tropica.
Sima Rostami, Reza Salavati, Robin N. Beech, Zahra Babaei, Mitra Sharbatkhori, Saeedeh Shamsaddini, Saeid Nasibi, and Majid Fasihi Harandi
Parasitology, ISSN: 00311820, eISSN: 14698161, Volume: 146, Pages: 563-568, Published: 1 April 2019 Cambridge University Press (CUP)
AbstractLittle is known about the genetic and morphological characters of Taenia ovis. The purpose of the present study was to characterize sheep isolates of T. ovis using rostellar hook morphometry as well as mitochondrial genes sequence analysis. Ninety sheep specimens of Cysticercus ovis were collected from 18 slaughterhouses in Iran. The mean ± s.d. for total length of large and small hooks were 174.1 ± 6.4 and 116.7 ± 5.4 µm, respectively. CO1 and 12S rRNA sequence analysis showed 11 and nine haplotypes, respectively. The level of pairwise nucleotide variations between individual haplotypes of CO1 and 12S rRNA genes were 0.3–1.1 and 0.2–1.0%, respectively. Level of nucleotide variation in CO1 and 12S rRNA between T. ovis haplotypes from present study and eight other Taenia species was found to be 11.3–17.8 and 5.3–16.3%, respectively. Phylogenetic analysis clustered all T. ovis isolates into a single clade comprised of the all CO1 and 12S rRNA haplotypes. CO1 nucleotide difference between T. ovis ovis and T. asiatica was 13.6% that is lesser than the corresponding difference between T. ovis ovis and T. ovis krabbei, warranting the designation of two separate species as T. ovis and T. krabbei. Interclass correlation coefficients showed that there was no significant association between rostellar hook length variation and the variability of the mitochondrial genes.
Farideh TOHIDI, Bahram KAZEMI, Mojgan BANDEHPOUR, Iraj SHARIFI, Mohammad Reza RABIEI, Ebrahim SAEDI DEZAKI, and Zahra BABAEI
Iranian Journal of Parasitology, ISSN: 17357020, eISSN: 2008238X, Pages: 111-119, Published: January-March 2019 Knowledge E
Background: This study was aimed to silencing the Nucleoside transporter 3 (NT3) permease nucleobases involved in the salvage pathway of Leishmania in order to disrupt purine nucleotide uptake in the parasite and consequently, destruction of the parasite. Methods: Overall, 502 bp fragment of the NT3 gene sequence was designed to produce an antisense transcript upon entry of the vector into the parasite. The NT3 construct was transfected into L. major promastigotes and NT3 gene expression was studied in vivo and in vitro conditions. Results: Relative expression NT3 gene in transgenic Leishmania was decreased in tenth day. The percentages and the number of amastigotes infected macrophages with transgenic L. major were less than infected macrophages with wild-type strain. Our results in two groups of BALB/c female mice (wild-type strain and mutant, n=4 each group) were showed that size and number of ulcers in BALB/c mice infected with transgenic Leishmania promastigotes were less than the BALB/c mice infected with wild-type parasites. Conclusion: The results indicate the use of antisense RNA reduces of NT3 gene expression in L. major. More studies are required to obtain a new approach for treating Leishmania infection.
Sima Rostami, Reza Salavati, Robin N. Beech, Zahra Babaei, Mitra Sharbatkhori, Saeedeh Shamsaddini, Saeid Nasibi, and Majid Fasihi Harandi
Parasitology, ISSN: 00311820, eISSN: 14698161, Published: 2019 Cambridge University Press (CUP)
Razieh Tavakoli Oliaee, Iraj Sharifi, Ali Afgar, Abdollah Jafarzadeh, Amir Tavakoli Kareshk, Mehdi Bamorovat, Hamid Sharifi, Zahra Babaei, Amir Keyhani, Alireza Keyhani, Leili Abedi, and Fatemeh Sharifi
Microbial Pathogenesis, ISSN: 08824010, eISSN: 10961208, Volume: 126, Pages: 368-378, Published: January 2019 Elsevier BV
BACKGROUND Detection of the mechanism of host/parasite interactions in unresponsive forms of anthroponotic cutaneous leishmaniasis (ACL) caused by Leishmania tropica is helpful for immunotherapy and vaccine development. In the present study, the gene expression of toll-like receptors (TLRs), TNF-α, iNOS and also arginase (ARG) activity in monocytes from Glucantime unresponsive in comparison to responsive patients infected with L. tropica was investigated. METHODS In this case-control study, patients with unresponsive (n = 10) and responsive (n = 10) ACL were recruited. Gene expression of TLR2, TLR4, TLR9, TNF-α and iNOS was analyzed in L. tropica-exposed monocytes. The level of ARG activity in both isolated promastigotes and the lysates of monocytes was also determined. RESULTS L. tropica-exposed monocytes represented higher expression of all three TLRs and TNF-α and lower expression of iNOS compared to unexposed ones in both groups of patients. Results revealed a significant down-regulation of TLR2 and TNF-α and up-regulation of TLR9 expression in unresponsive isolates in comparison to responsive ones. Besides, ARG level showed a significant increase in L. tropica-stimulated monocytes and cultured promastigotes from unresponsive isolates versus responsive ones. CONCLUSIONS The decreased TLR2, TLR4, TNF-α and iNOS and the increased level of TLR9 expression in L. tropica-exposed monocytes from unresponsive isolates and also the increment in ARG activity in their promastigotes and monocytes, might possibly be involved in the severity of the disease and leading to Glucantime unresponsiveness.
A. Askari, I. Sharifi, M.R. Aflatoonian, Z. Babaei, P. Ghasemi Nejad Almani, M.A. Mohammadi, H. Alizadeh, S. Hemati, and M. Bamorovat
Microbial Pathogenesis, ISSN: 08824010, eISSN: 10961208, Volume: 121, Pages: 363-368, Published: August 2018 Elsevier BV
Leishmaniasis is rising in many countries, including Iran, due to climate change, refugee crises, urbanization and etc. The aim of this study was to explore the epidemiology, extent and identity of Leishmania species in a newly emerged focus in Abdanan County, Ilam Province, South-western Iran. This study was performed as a descriptive cross-sectional study by a systematic house-to-house approach. The Leishmania species was identified by RFLP-PCR and sequencing. Altogether, 46799 individuals consisting of 22907 (48.9) female and 23892 (51.1%) male were interviewed and physically examined for the presence of skin lesions. Overall, the incidence rate was 0.34% (n = 160). All age groups were affected and the incidence rate was the highest in <10 years of age group (0.49%) and the lowest in >50 years old individuals (0.15%), although there was no significant difference regarding the sex and age. The majority of patients had one lesion (47.5%) on hands (56%) and most of the cases occurred in Abdanan city (%54) in summer. Based on the RFLP-PCR analysis, all the Leishmania isolates were L. major of single genotype. A newly emerged focus of zoonotic CL caused by L. major occurred in South-western of Iran. Multiple risk factors created this epidemic area. Further studies on the vector and reservoir are crucial needed to provide evidences to select the prophylactic and therapeutic measures for future control strategies.
Maryam Ramezany, Iraj Sharifi, Zahra Babaei, Pooya Ghasemi Nejad Almani, Amireh Heshmatkhah, Alireza Keyhani, Mohammad Reza Aflatoonian, Mohammad Ali Mohammadi, Fatemeh Sharifi, and Mehdi Bamorovat
Pathogens and Global Health, ISSN: 20477724, eISSN: 20477732, Volume: 112, Pages: 132-141, Published: 3 April 2018 Informa UK Limited
This study aimed to explore geographic distribution and molecular characterization of cutaneous leishmaniasis (CL) species by amplifying two popular markers in kinetoplast DNA and internal transcribed spacer 1 loci by nested-PCR, and characterized by sequencing and phylogenetic analyses. Findings demonstrated that two species co-existed in the province: L. tropica (88.5%) and L. major (11.5%). All gender and age groups were equally infected, although males, 21-30 years old, exhibited a significantly higher infection. Sequencing and phylogenetic analyses of 34 randomly selected samples showed that L. tropica isolates exhibited some degree of heterogeneity. Both anthroponotic CL and zoonotic CL are present in south-eastern Iran with predominance of L. tropica species. Some level of heterogeneity was observed in L. tropica isolates which possibly reflects different colonies in the area. Implementation of diagnostic tools directly from clinical samples could be an important strategic approach for exploration of spatial distribution, molecular characterization and phylogenetic analyses.
Maryam Nooshadokht, Behjat Kalantari-Khandani, Iraj Sharifi, Hossein Kamyabi, Namal P.M. Liyanage, Laurel A. Lagenaur, Martin F. Kagnoff, Steven M. Singer, Zahra Babaei, and Shahram Solaymani-Mohammadi
Journal of Microbiological Methods, ISSN: 01677012, eISSN: 18728359, Volume: 141, Pages: 35-41, Published: October 2017 Elsevier BV
Human infection with the protozoan parasite Giardia duodenalis is one the most common parasitic diseases worldwide. Higher incidence rates of giardiasis have been reported from human subjects with multiple debilitating chronic conditions, including hypogammaglobulinemia and common variable immunodeficiency (CVID). In the current study, stool specimens were collected from 199 individuals diagnosed with HIV or cancer and immunocompetent subjects. The sensitivity of microscopy-based detection on fresh stool preparations, trichrome staining and stool antigen immunodetection for the diagnosis of G. duodenalis were 36%, 45.5% and 100%, respectively when compared with a highly sensitive stool-based PCR method as the gold standard. Further multilocus molecular analyses using glutamate dehydrogenase (gdh) and triose phosphate isomerase (tpi) loci demonstrated that the AI genotype of G. duodenalis was the most prevalent, followed by the AII genotype and mixed (AI+B) infections. We concluded that stool antigen immunodetection-based immunoassays and stool-based PCR amplification had comparable sensitivity and specificity for the diagnosis of G. duodenalis infections in these populations. Stool antigen detection-based diagnostic modalities are rapid and accurate and may offer alternatives to conventional microscopy and PCR-based diagnostic methods for the diagnosis of G. duodenalis in human subjects living with HIV or cancer.
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 251-259, Published: 2017
Tropical Biomedicine, ISSN: 01275720, Pages: 681-690, Published: 2017
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 1-11, Published: 2017
Zahra Babaei, Najmeh Malihi, Naser Zia-Ali, Iraj Sharifi, Mohammad A. Mohammadi, Martin F. Kagnoff, Lars Eckmann, Steven M. Singer, and Shahram Solaymani-Mohammadi
Microbes and Infection, ISSN: 12864579, eISSN: 1769714X, Pages: 687-695, Published: 1 November 2016 Elsevier BV
The genetic basis of the ultimate clinical outcomes of human giardiasis has been the subject of numerous investigations. We previously demonstrated roles for both host and parasite factors in determining the outcome of enteric infection in a murine model of Giardia duodenalis infection. In the current study, fecal and serum specimens from healthy controls and human subjects infected with the intestinal parasite G. duodenalis were assessed. Using a semi-nested PCR method, clinical isolates were genetically characterized based on the gdh and tpi loci, and the phylogenetic trees were constructed. Using a sandwich ELISA method, the serum levels of representative TH1 and TH2 cytokines were measured in infected human subjects and healthy controls. Here we showed that symptomatic human giardiasis was characterized by significantly elevated serum levels of the TH1 cytokine IFN-γ compared to healthy controls, whereas asymptomatic human subjects and healthy controls had comparable levels of serum IFN-γ. Further analyses showed that human subjects infected with G. duodenalis genotype AI had significantly elevated levels of serum IFN-γ and IL-10, but not IL-5, whereas human subjects infected with AII had similar levels of those cytokines compared to healthy controls. These data demonstrate roles for both host and parasite factors in the determination of the outcome of enteric infections and may further broaden our understanding of host-parasite interaction during enteric protozoal infections.
Pooya Ghasemi Nejad Almani, Iraj Sharifi, Bahram Kazemi, Zahra Babaei, Mojgan Bandehpour, Samira Salari, Ebrahim Saedi Dezaki, Farideh Tohidi, and Mohammad Ali Mohammadi
Acta Tropica, ISSN: 0001706X, eISSN: 18736254, Volume: 154, Pages: 63-72, Published: February 01, 2016 Elsevier BV
At present, there are no efficacious vaccines or effective drugs against leishmaniasis; therefore new and innovative control methods are urgently required. One way to achieve this important goal is through using reverse genetic engineering to evaluate important enzymes, proteins and macromolecules. One of the most important enzymes for Glycosylphosphatidylinositol (GPI) biosynthetic pathways is GlcNAc-PI-de-N-acetylase (GPI12). The molecular constructs were cloned in Escherichia coli strain Top 10 and confirmed by molecular methods and were transfected by electroporation into Leishmania major. We demonstrated that two alleles of the GPI12 gene in L. major were successfully removed and enabling the generation of a null mutant, which supports the idea that GPI12 is not an essential gene for the growth and survival of Leishmania and the homozygous knockouts of Leishmania are able to survive. We were able to produce a mutant parasite that caused no damaged to the host. Further investigations are essential to check the safety profile in laboratory animals.
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 290-295, Published: 2016
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 448-462, Published: 2016
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 625-631, Published: October-December 2015
Sima Rostami, Reza Salavati, Robin N. Beech, Zahra Babaei, Mitra Sharbatkhori, and Majid Fasihi Harandi
Parasitology Research, ISSN: 09320113, eISSN: 14321955, Volume: 114, Pages: 1365-1376, Published: 19 March 2015 Springer Science and Business Media LLC
Taenia saginata is an important tapeworm, infecting humans in many parts of the world. The present study was undertaken to identify inter- and intraspecific variation of T. saginata isolated from cattle in different parts of Iran using two mitochondrial CO1 and 12S rRNA genes. Up to 105 bovine specimens of T. saginata were collected from 20 slaughterhouses in three provinces of Iran. DNA were extracted from the metacestode Cysticercus bovis. After PCR amplification, sequencing of CO1 and 12S rRNA genes were carried out and two phylogenetic analyses of the sequence data were generated by Bayesian inference on CO1 and 12S rRNA sequences. Sequence analyses of CO1 and 12S rRNA genes showed 11 and 29 representative profiles respectively. The level of pairwise nucleotide variation between individual haplotypes of CO1 gene was 0.3–2.4 % while the overall nucleotide variation among all 11 haplotypes was 4.6 %. For 12S rRNA sequence data, level of pairwise nucleotide variation was 0.2–2.5 % and the overall nucleotide variation was determined as 5.8 % among 29 haplotypes of 12S rRNA gene. Considerable genetic diversity was found in both mitochondrial genes particularly in 12S rRNA gene.
Sima Rostami, Shahriar Dabiri, Zahra Babaei, Majid Fasihi Harandi, Shams Shariat Torbaghan, Mohammad Ali Mohammadi, and Mitra Sharbatkhori
American Journal of Tropical Medicine and Hygiene, ISSN: 00029637, Pages: 588-594, Published: 1 March 2015 American Society of Tropical Medicine and Hygiene
Cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus, presents an important medical and veterinary problem globally, including that in Iran. Different genotypes of E. granulosus have been reported from human isolates worldwide. This study identifies the genotype of the parasite responsible for human hydatidosis in three provinces of Iran using formalin-fixed paraffin-embedded tissue samples. In this study, 200 formalin-fixed paraffin-embedded tissue samples from human CE cases were collected from Alborz, Tehran, and Kerman provinces. Polymerase chain reaction amplification and sequencing of the partial mitochondrial cytochrome c oxidase subunit 1 gene were performed for genetic characterization of the samples. Phylogenetic analysis of the isolates from this study and reference sequences of different genotypes was done using a maximum likelihood method. In total, 54.4%, 0.8%, 1%, and 40.8% of the samples were identified as the G1, G2, G3, and G6 genotypes, respectively. The findings of the current study confirm the G1 genotype (sheep strain) to be the most prevalent genotype involved in human CE cases in Iran and indicates the high prevalence of the G6 genotype with a high infectivity for humans. Furthermore, this study illustrates the first documented human CE case in Iran infected with the G2 genotype.
S. Rostami, R. Salavati, R.N. Beech, Z. Babaei, M. Sharbatkhori, M.R. Baneshi, E. Hajialilo, H. Shad, and M.F. Harandi
Journal of Helminthology, ISSN: 0022149X, eISSN: 14752697, Pages: 150-157, Published: 8 March 2015 Cambridge University Press (CUP)
AbstractAlthough Taenia hydatigena is one of the most prevalent taeniid species of livestock, very little molecular genetic information exists for this parasite. Up to 100 sheep isolates of T. hydatigena were collected from 19 abattoirs located in the provinces of Tehran, Alborz and Kerman. A calibrated microscope was used to measure the larval rostellar hook lengths. Following DNA extraction, fragments of cytochrome c oxidase 1 (CO1) and 12S rRNA genes were amplified by the polymerase chain reaction method and the amplicons were subjected to sequencing. The mean total length of large and small hooks was 203.4 μm and 135.9 μm, respectively. Forty CO1 and 39 12S rRNA sequence haplotypes were obtained in the study. The levels of pairwise nucleotide variation between individual haplotypes of CO1 and 12S rRNA genes were determined to be between 0.3–3.4% and 0.2–2.1%, respectively. The overall nucleotide variation among all the CO1 haplotypes was 9.7%, and for all the 12S rRNA haplotypes it was 10.1%. A significant difference was observed between rostellar hook morphometry and both CO1 and 12S rRNA sequence variability. A significantly high level of genetic variation was observed in the present study. The results showed that the 12S rRNA gene is more variable than CO1.
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 342-349, Published: 2014
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 530-535, Published: 2013
Sima Rostami, Reza Salavati, Robin N. Beech, Mitra Sharbatkhori, Zahra Babaei, Sadegh Saedi, and Majid Fasihi Harandi
Veterinary Parasitology, ISSN: 03044017, eISSN: 18732550, Volume: 197, Issue: 1-2, Pages: 141-151, Published: 18 October 2013 Elsevier BV
Taenia multiceps is a widely distributed zoonotic tapeworm of canids. The larval stage of the parasite (Coenurus) occurs in sheep, goat and cattle and has been rarely reported from humans. This study investigated genetic variability of two mitochondrial genes in 102 isolates of T. multiceps. Metacestodes were collected from brains and hearts of sheep in Tehran and Qom provinces of Iran. DNA of each isolate was extracted and used for PCR amplification of cytochrome c oxidase subunit I (CO1) and 12S ribosomal DNA (12S rRNA) genes. All amplicons were sequenced and the sequence data were analyzed using NCBI Blast and BioEdit. Phylogenetic trees and pairwise calculations were obtained by using Mega5 software. In total 7 and 25 representative haplotypes were differentiated for CO1 and 12S rRNA genes, respectively. For CO1 sequences 11 segregation sites within 7 haplotypes were observed. For 12S rRNA sequences a total of 32 segregation sites were observed in 25 haplotypes. CO1 gene displayed lower diversity than 12S rRNA gene with an overall nucleotide variation of 3.0% for CO1 vs. 7.2% for 12S rRNA. Pairwise comparisons among 7 haplotypes in CO1 and 12S rRNA genes showed the level of nucleotide differences 0.3-2.5% and 0.2-4.0%, respectively. A high degree of genetic variation was found in the isolates of T. multiceps in Iran. Additional molecular studies are required on the parasite from other intermediate hosts.
Sima Rostami, Saeed Talebi, Zahra Babaei, Mitra Sharbatkhori, Naser Ziaali, Habib Rostami, and Majid Fasihi Harandi
Parasitology Research, ISSN: 09320113, eISSN: 14321955, Volume: 112, Pages: 3441-3447, Published: October 2013 Springer Science and Business Media LLC
Reliable and rapid genotyping of large number of Echinococcus granulosus sensu lato isolates is crucial for understanding the epidemiology and transmission of cystic echinococcosis. We have developed a method for distinguishing and discriminating common genotypes of E. granulosus s.l. (G1, G3, and G6) in Iran. This method is based on polymerase chain reaction coupled with high resolution melting curve (HRM), ramping from 70 to 86 °C with fluorescence data acquisition set at 0.1 °C increments and continuous fluorescence monitoring. Consistency of this technique was assessed by inter- and intra-assays. Assessment of intra- and inter-assay variability showed low and acceptable coefficient of variations ranging from 0.09 to 0.17 %. Two hundred and eighty E. granulosus s.l. isolates from sheep, cattle, and camel were used to evaluate the applicability and accuracy of the method. The isolates were categorized as G1 (93, 94, and 25 %), G3 (7, 4, and 4 %), and G6 (0, 2, and 71 %) for sheep, cattle, and camel, respectively. HRM results were completely compatible with those obtained from sequencing and rostellar hook measurement. This method proved to be a valuable screening tool for large-scale molecular epidemiological studies.
Life Science Journal, ISSN: 10978135, Issue: SUPPL.3, Pages: 373-377, Published: 2013
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 99-102, Published: 2012
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 10-15, Published: 2012
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 53-58, Published: 2012
Journal of Mazandaran University of Medical Sciences, ISSN: 17359279, eISSN: 17359260, Pages: 38-46, Published: 2012
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 1-7, Published: 2011
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 1-7, Published: 2011
Zahra Babaei, Hormozd Oormazdi, Sasan Rezaie, Mostafa Rezaeian, and Elham Razmjou
Experimental Parasitology, ISSN: 00144894, eISSN: 10902449, Volume: 128, Pages: 159-162, Published: June 2011 Elsevier BV
Difficulty in disrupting cysts of Giardia intestinalis, a cosmopolitan protozoan parasite, decreases the yield of DNA extracted and reduces the effectiveness of the polymerase chain reaction (PCR). To improve the detection of the Giardia Glutamate Dehydrogenase (gdh) gene, we re-evaluated the effects of deoxyribonucleic acid (DNA) extraction methods. Purified and concentrated cysts from 33 fecal samples were disrupted using conventional methods, and DNA extraction was conducted using two protocols: the QIAamp Stool Mini Kit and phenol/chloroform/isoamyl alcohol (PCI). PCR amplification was successful for 12 extracted DNA samples (36%) using PCI following a glass bead and freeze/thaw pretreatment and for all 33 samples (100%) using the QIAamp Stool Mini Kit following the aforementioned pretreatment. Consequently, the pretreatment of cysts with glass beads and freeze/thaw cycles followed by extraction of DNA with the QIAamp Stool Mini kit was the more effective protocol.
Iranian Journal of Public Health, ISSN: 22516085, eISSN: 22516093, Pages: 80-85, Published: 2011
Mansoureh Vatanshenassan, Sassan Rezaie, Mehdi Mohebali, Nasrin Niromand, Bahram Kazemi, Zahra Babaei, and Mostafa Rezaeian
Experimental Parasitology, ISSN: 00144894, eISSN: 10902449, Volume: 126, Pages: 187-190, Published: October 2010 Elsevier BV
Trichomonas vaginalis is the agent of a highly prevalent sexually transmitted disease that leads to vaginitis, urethritis, ectocervicitis and has been associated with human immunodeficiency virus (HIV). Detection of T. vaginalis based on wet-mount microscopy and culture methods is insensitive and time consuming, respectively. Thus the quest for reliable PCR techniques of T. vaginalis in vaginal discharge and urine sample is more importance. In this study, 500 urine and vaginal-discharge samples were collected from women referred to Sexual Transmitted Disease Clinic of Mirzakuchakkhan Hospital in Tehran, Iran between May 2008 and March 2009. Wet-mount and culture methods were done on the vaginal discharges, and PCR assay targeting cysteine proteinase 4 (CP4) was performed on the urine samples. The present study demonstrated 16 (3.2%) of patients were infected with T. vaginalis using culture and wet-mount, whereas PCR assay using CP4 could detect 12 (2.4%) positivity. Sensitivity and specificity of urine PCR assay compared to culture were 80% (95% CI, 54-96) and 99.6% (95% CI, 98.96-100), respectively. These results indicate that using urine-based detection method for T. vaginalis may not be appropriate in women.
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 1-5, Published: 2010
Iranian Journal of Public Health, ISSN: 22516085, eISSN: 22516093, Pages: 64-69, Published: 2010
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 43-47, Published: 2009
Iranian Journal of Parasitology, ISSN: 17357020, Pages: 38-43, Published: 2009
Iranian Journal of Public Health, ISSN: 22516085, eISSN: 22516093, Pages: 75-82, Published: 2008
S. Solaymani-Mohammadi, M. Rezaian, Z. Babaei, A. Rajabpour, A. R. Meamar, A. A. Pourbabai, and W. A. Petri
Journal of Clinical Microbiology, ISSN: 00951137, Pages: 2258-2261, Published: June 2006 American Society for Microbiology
The present study was conducted to compare stool antigen detection with PCR for the diagnosis of Entamoeba sp. infection in asymptomatic cyst passers from Iran. Entamoeba dispar and, in one case, E. moshkovskii were the Entamoeba spp. found in the amebic cyst passers. There was a 100% correlation between the results from the TechLab E. histolytica II stool antigen kit and those from nested PCR. We concluded that E. dispar is much more common in asymptomatic cyst passers in Iran and that antigen detection and PCR are comparable diagnostic modalities.
S. Solaymani-Mohammadi, M. Rezaian, H. Hooshyar, G. R. Mowlavi, Z. Babaei, and M. A. Anwar
Journal of Wildlife Diseases, ISSN: 00903558, Pages: 801-803, Published: October 2004 Wildlife Disease Association
A total of 12 gastrointestinal tracts of wild boars (Sus scrofa) from western Iran (Luristan) were examined for protozoan infection between September 2000 and November 2001. Of 12 boars examined, 67% harbored one or more species of the following protozoa: Balantidium coli (25%), Tritrichomonas suis (25%), Blastocystis sp. (25%), Entamoeba polecki (17%), Entamoeba suis (8%), Iodamoeba butschlii (17%), and Chilomastix mesnili (8%). Four of these protozoan species also are reported in humans, and persons living in rural areas where wild boars are abundant should take precaution to avoid infection.