Elena Petrovna Sokolik
@onmedu.edu.ua
pharmacology and pharmacognosy
Odessa State Medical University
EDUCATION
Zaporozhye state medical university: Zaporozhye, Zaporozhye, UA
RESEARCH INTERESTS
neuroprotection, pharmacology, medicine, biochemistry
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Olena Petrivna Sokolik and Galina Olexandrivna Prozorova
Neurotak Publishing
One of the promising therapy areas for many diseases of the central nervous system is the search for agents of selective effect on mitochondria. Both the mitochondria themselves and the mitochondrial metabolism of the transformed cell of the central nervous system and activation of energy metabolism by reprogrammed mitochondria give impetus for the development of mitochondrial pharmacology to use the special properties of transformed cells mitochondria as targets for neuroprotective and neuroplastic effects. In this review, we analyse literary sources of domestic and foreign authors about the influence of mitochondrial dysfunction on various links in the pathogenesis of central nervous system diseases. Based on currently available data, scientists divided all signs of mitochondrial dysfunction in schizophrenia into three groups: morphological disorders of mitochondria, signs of a violation of the oxidative phosphorylation system and dysregulation of genes responsible for mitochondrial proteins. The therapeutic effect of drugs for central nervous system disorders should focus on reducing the accumulation of metabolic products and tissue breakdown, restoring mitochondrial functions and synaptic plasticity, and protecting mitochondria from toxic effects, thereby alleviating cognitive disorders with a neuroprotective effect.
Olena P. Sokolik and Galina O. Prozorova
Pensoft Publishers
Introduction: Fibrocystic breast disease, commonly called fibrocystic breasts or fibrocystic change, is a benign (noncancerous) condition, which is the most common pathology in women of reproductive age. Treatment of fibrocystic breast disease and concomitant pathologies can involve using herbs. Materials and Methods: To make an analysis of literary sources on the development of fibrocystic breast disease in the pathogenesis of diseases of the female reproductive system (clinical human (75%) and animal studies (25%)) were published in the period of 2017–2021. Results and discussion: The diversity of plants in the world is a promising ground for therapeutic improvisation, allowing for an individual approach to each patient, but, most importantly, creates possibilities for maneuvering in the event of ineffectiveness of any means. In some situations, herbal medicine is not only possible or permissible, but strictly mandatory, and is essentially the only effective therapeutic method, which is relatively safe provided the correct selection of combinations and control by a doctor who applies a certain method of phytotherapy, especially given a duration of treatment. The need for a deeper study is long overdue for the pharmacological capabilities of various plant raw materials in the treatment of not only this pathology, but others as well. Conclusion: The development of phytotherapy should be based primarily on scientific developments, but this area can not be considered the prerogative of only phytotherapists, as herbal medicines should be in the arsenal of doctors of all specialties.
Igor F. Belenichev, Elena P. Sokolik, Nina V. Bukhtiarova, and Sergii V. Levich
Informa UK Limited
Objective: One of the primary reactions of the genome in response to stress is different genesis induction of heat shock proteins – HSP. The purpose of this study was to investigate the concentration of heat shock protein (HSP70) and hypoxia-inducible factor (HIF-1) in the brain of rats undergoing chronic prenatal alcoholism in different periods of ischemia and define the role of these proteins in the implementation of neuroprotective effect of Cerebrocurin and Tiocetam.
Methods: Experiments were carried out on female rats weighing 150-180 g. All animals were on standard food ration of vivarium, with natural alteration of day and night. Rats were recieved from nursery of «Institute of Pharmacology and Toxicology, Academy of Medical Sciences of Ukraine». All experimental procedures and operative interventions were done in accordance with WMA Statement on Animal Use in Biomedical Research. Rats from the 5th to the 20th day of gestation received ethanol in a dose of 6-8 g/kg/day, control rats – isocalorific sucrose solution. Offspring of alcoholized rats immediately after birth during 25 days were injected intraperitoneally Tiocetam (125 mg/kg), Piracetam (125 mg/kg) and Cerebrocurin (0.06 mg/kg), control rats received saline solution. There were 20 infants in each group. Biochemical studies carried out on brain on 26 days of the experiment, for this purpose the animals were decapitated under anesthesia using Thiopental (30 mg/kg, intraperitoneally). Concentration in the brain tissue and HIF proteins and HSP proteins were determined by Western blot analysis.
Results: Study of concentration in brain tissue HIF proteins and HSP-proteins showed that after undergoing prenatal chronic alcoholism there was an observed decrease in concentration of HSP, so HIF-proteins. Course treatment by Cerebrocurin and Tiocetam resulted in statistically significant increased content of HIF and HSP proteins in the brain in comparison with a group of untreated animals. Neuroprotective activity of Cerebrocurin and Tiocetam was observed in reduction of neurological deficit, as evidenced by the statistically significant decrease in the average score on a scale of C.P. McGrow. Cerebrocurin and Tiocetam directly or indirectly can modulate the expression of early response c-fos genes and thus the “run” software adaptation protein synthesis (including HSP and HIF) in neurons with acute cerebral ischemia.
Conclusion: Implementation of the neuroprotective effect of Cerebrocurin and Tiocetam revealed apparently their ability to increase the concentration in the brain tissues of HSP-protein.
I. F. Belenichev, Yu. M. Kolesnik, S. V. Pavlov, E. P. Sokolik, and N. V. Bukhtiyarova
Pleiades Publishing Ltd
Acute or chronic brain ischemia induces a cascade of pathobiochemical reactions that finally result in the development of focal neurological deficit, dyscirculatory encephalopathy, or the death of a patient. We studied the effects of ischemia at different time points, including 1, 6, 24, 48, 72, and 120 h, and 21 days. During the period of the strongest ischemia-induced disturbances (24–72 h), we found lactate over-production associated with inhibition of hexokinase, an enzyme that catalyzes the first “trigger” reaction of glycolysis. An increase in the malate content associated with increasing activities of mitochondrial and cytosolic malate dehydrogenases within the first hours of cerebral ischemia indicates the activation of the malateaspartate shuttle, which is responsible for the transportation of reduced equivalents to mitochondria. The inhibition of malate production and activity of NAD-dependent malate dehydrogenase correlates with a decrease in the contents of ATP, HSP70, and hypoxia-induced factor-1a (HIF-1a) and the severity of neurological disturbances. We believe that in response to brain ischemia, HIF-1a is expressed, which induces compensatory mechanisms of energy production.
I. F. Belenichev, Yu. M. Kolesnik, S. V. Pavlov, E. P. Sokolik, and N. V. Bukhtiyarova
Pleiades Publishing Ltd
We studied the involvement of HSP70 and Hif1b genes in the development of pathological changes in the brains of experimental animals during ischemia. We found neuro- and mitoprotective characteristics of heat shock protein HSP70 and Hif1b in vitro and under cerebral ischemia due to stabilization of molecules damaged by oxidation, inhibition of nitrosative and oxidative stress and mitoprotective action directed to amelioration of mitochondrial dysfuction. The protective role of these proteins provides a new direction for the development of novel neuroprotective drugs that protect or modulate the genes that encode these proteins.